سامانه همایش های دانشگاه آزاد اسلامی واحد نجف آباد

صفحه اصلی / اولین همایش بین المللی تحول خدمات سلامت: تلفیق مراقبت، درمان، بازتوانی و امیدبخشی

Positive Correlation Between miR-16-5p Expression and VEGF Protein Levels in the Endometrium of Progesterone-Treated Mice Immediately Before Implantation

نویسندگان :

Bahman Rashidi¹ Mohammad Zamani Rarani² Fahimeh Zamani Rarani³

1- گروه علوم تشریحی و بیولوژی تولید مثل، دانشکده ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران 2- گروه علوم تشریحی، دانشکده ی پزشکی، دانشگاه علوم پزشکی هرمزگان، بندرعباس، ایران 3- گروه علوم تشریحی و بیولوژی تولید مثل، دانشکده ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

کلمات کلیدی :

Endometrium،MicroRNAs،Uterus،Vascular Endothelial Growth Factors،Progesterone

چکیده :

Background: Angiogenesis-related microRNAs regulate the expression of genes responsible for blood vessel formation, including Vascular Endothelial Growth Factor (VEGF). Angiogenesis is indispensable during pregnancy, as it supports development of a receptive endometrial environment for embryo implantation. Progesterone plays a role in regulating endometrial transformation and promoting angiogenic processes during this period (1-3). This study aimed to examine the relationship between miR-16-5p expression and VEGF levels in the pre-implantation endometrium of mice receiving exogenous progesterone. Methods: Twenty adult female mice were divided into two groups: 1) control and 2) progesterone (1 mg/mouse of progesterone intraperitoneally for 3 consecutive days, 72 hours after day 0) (4, 5). Pearson's correlation analysis was performed to evaluate the association between endometrial miR-16-5p expression, quantified by real-time PCR, and VEGF protein levels, determined by immunohistochemistry (IHC) and Western blot analysis. Findings: Regarding IHC data, miR-16-5p showed a strong positive correlation with VEGF protein expression (r = 0.74, p<0.05 in the control group; r = 0.78, p<0.01 in the progesterone-treated group). Similarly, Western blot analysis confirmed a significant positive correlation between miR-16-5p expression and VEGF protein levels. Conclusion: miR-16-5p may play a role in regulating endometrial angiogenesis through its association with VEGF during the pre-implantation period in the presence of exogenous progesterone.